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P.C.R

Amplification of DNA

PCR involves 30-60 cycles to amplify DNA sufficiently as

each cycle doubles the DNA.
 

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Practical Applications

 Help solve crimes
 
 
 


Paternity Testing

  

 

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Requirements for PCR

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1.  Target DNA sample
2. Primers
3. ATP
4. Heat tolerant DNA polymerase
5. free DNA nucleotides

** Ligase not needed**
As there is no lagging strand.

DNA polymerase - heat tolerant
To prevent enzyme being denatured at
high temperatures of PCR.

 

Stages of PCR

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Each cycle involves 3 stages:

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1.  heating to 92-98 degrees

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2. cooling to 50-65 degrees

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3. heating to 70-80 degrees

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​To separate the DNA strands.

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1. For primers to bind to target DNA

at 3' end.

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​2. To start DNA replication by

DNA polymerase​​​.

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For DNA polymerase to replicate the DNA​.

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